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Avian influenza virus inspection report
41℃ soluble collagen / 2021-01-20
  • Although Taiwan is not an avian influenza epidemic area, every batch of raw materials produced by the company has passed the Avian Influenza PCR Test (Avian Influenza PCR Test).
    Use it only if it is confirmed as a negative result (no virus reaction). The quality is guaranteed, please feel free to buy and use.
    Avian influenza virus inspection report
    Detection method: Avian Influenza PCR Test
    Sample name: 41℃ Soluble Collagen
    Sample batch number: 921002-2-1
    Measure the number of samples: S1 and S2 are measured twice for the same sample
    recording:

 

 

  

 

 

 

 

 

 

 

 

  • M = Marker (standard base, containing known bases of different sizes, for comparison purposes)
    P = Positive Control, used for control N = Negative Control, used for control
    S1 = Sample 1
    S2 = Sample 2
    test result:
    Both S1 and S2 samples were negative.

 


Dr. Collagen explained as follows:

 

 

 

1. What is PCR

 

 

 

PCR is a term for a method to test genes, and its full name is Polymerase Chain Reaction. This PCR detection method can be directly used to determine the absence and presence of the certain gene (DNA or RNA).

 

 

 

2. The principle of PCR

 

 

 

a) Copy

 

PCR is currently one of the most sensitive and accurate test methods in the world. Its principle is to copy the test object 50 or 80 times (this part can be adjusted), and then we  take the copied test object to do electrophoresis analysis, you can directly interpret the results. Why copy 50 or 80 times?

 

 

 

Let me give an example to illustrate that if only one virus exists in the test object,

 

One copy becomes two,

 

Copy 5 times to become 32,

 

Copying 10 times becomes 1024,

 

Copying 15 times becomes 32768,

 

Copying 20 times becomes 1048576,

 

Copying 25 times becomes 33554432,

 

Copying 30 times becomes 1073741824,

 

Copy 35 times to become 34359738368,

 

Sorry! The number is too big, my computer crashes, it doesn't count.

 

 

 

b) Electrophoresis analysis

 

 

Then, the copied test object to do further electrophoresis analysis, and the result is directly interpreted.

 

 

 

The description of this electropherogram is as follows

 

 

 

 

 

 

 

 

 

 

 

Interpretation results:


Looking down from P, you can see a white band, which is a positive reaction, indicating that the standard positive solution is qualified.


Looking down from N, there is no (no) white band, which is a negative reaction, indicating that the standard negative solution is qualified.


Looking down from S1, there is no (no) white band, which is a negative reaction.


Looking down from S2, there is no (no) white band, which is a negative reaction.